Name |
JAE-YOUNG YUN |
MAJOR |
Life Science (CRISPR genome editing) |
TEL |
02-970-6239 |
E-mail |
jae0yun@seoultech.ac.kr |
Biography
2010 Ph.D. in Biochemistry, University of Wisconsin-Madison, USA
2002 M.S. in Developmental Genetics, Seoul National University
2000 B.S. Dept. of Biology, Seoul National University
2002 M.S. in Developmental Genetics, Seoul National University
2000 B.S. Dept. of Biology, Seoul National University
Careers
2023~ Associate Professor, School of Convergence, Seoul National University of Science and Technology
2021-2023 Principal Investigator, Dept. of Life Science, Sogang University
2020-2021 Senior Researcher, Institutes of GBST, Seoul National University
2014-2020 Research Fellow, Center for Genome Engineering, Institute for Basic Science
2014-2014 Visiting Scholar, Center for Algae Biotechnology, UCSD, USA
2013-2014 Research Scholar, Molecular and Computational Biology, University of Southern California, USA
2012-2013 Research Associate, Salk Institute for Biological Studies, USA
2011-2011 Postdoctoral Fellow, University of Wisconsin-Madison, Madison, WI, USA
2021-2023 Principal Investigator, Dept. of Life Science, Sogang University
2020-2021 Senior Researcher, Institutes of GBST, Seoul National University
2014-2020 Research Fellow, Center for Genome Engineering, Institute for Basic Science
2014-2014 Visiting Scholar, Center for Algae Biotechnology, UCSD, USA
2013-2014 Research Scholar, Molecular and Computational Biology, University of Southern California, USA
2012-2013 Research Associate, Salk Institute for Biological Studies, USA
2011-2011 Postdoctoral Fellow, University of Wisconsin-Madison, Madison, WI, USA
Research Areas
*RESEARCH INTERESTS
- Studies of genetic/epigenetic contributions to natural variations
- Development of the CRISPR systems for plant genome editing
- Development of the high-throughput platforms for CRISPR library screen
*TECHNICAL EXPERTISE
- CRISPR works: CRISPR/Cas9, CRISPR base editors, CRISPR prime editors, X-MAS system
- Nucleic acid works: HTP DNA/RNA isolation, real-time quantitative PCR, TAIL-PCR, 5’/3’ RACE-PCR, degenerate PCR
- Protein works: Western blot, SDS-PAGE, Chromatin IP
- Genomics works: NGS analyses, Development of genotyping markers/systems, Development of HTP screen systems
- in vivo studies: Arabidopsis, Radish, Banana, Chlamydomonas, Yeast, Tardigrade, Axolotl, Zebrafish, and Mouse
- Studies of genetic/epigenetic contributions to natural variations
- Development of the CRISPR systems for plant genome editing
- Development of the high-throughput platforms for CRISPR library screen
*TECHNICAL EXPERTISE
- CRISPR works: CRISPR/Cas9, CRISPR base editors, CRISPR prime editors, X-MAS system
- Nucleic acid works: HTP DNA/RNA isolation, real-time quantitative PCR, TAIL-PCR, 5’/3’ RACE-PCR, degenerate PCR
- Protein works: Western blot, SDS-PAGE, Chromatin IP
- Genomics works: NGS analyses, Development of genotyping markers/systems, Development of HTP screen systems
- in vivo studies: Arabidopsis, Radish, Banana, Chlamydomonas, Yeast, Tardigrade, Axolotl, Zebrafish, and Mouse
Teaching
- Biotechnology and Society
- Genome and Understanding of Life
- Genome and Understanding of Life
Selected Publications
for the full updated list
◾ Lee J, Oh N, Yu SI, Yun JY*, Choi HS, Seo JK, Kang JH, and Jung C. Application of CRISPR-Based C-to-G Base editing in rice protoplasts. Applied Biological Chemistry (2023). https://doi.org/10.1007/s12374-022-09368-z (*Co-first author)
◾ Yun JY*, Yu SI, Bang SE, Kim JY, Lee S, and BH Lee. Identification of CRISPR-Induced Mutations in Plants: with a Focus on the Next-Generation Sequencing Assay. Journal of Plant Biology (2022). https://doi.org/10.1007/s12374-022-09368-z
◾ Park ME, Yun JY*, and Kim HU. C-to-G Base Editing Enhances Oleic Acid Production by Generating Novel Alleles of FATTY ACID DESATURASE 2 in Plants. Frontiers in Plant Science (2021). https://doi.org/10.3389/fpls.2021.748529 (*Co-corresponding author)
◾ Choi M, Yun JY*, Kim JH, Kim JS and Kim ST. The efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana. Scientific Reports (2021). https://doi.org/10.1038/s41598-021-87669-y (*Co-first author)
◾ Hu J, Cai J, Park SJ, Lee K, Li Y, Chen Y, Yun JY, Xu T, and Kang H. N6-Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis. Plant Journal (2021). https://doi.org/10.1111/tpj.15270
◾ Zhang J, Eswaran G, Alonso-Serra J, Kucukoglu M, Xiang J, Yang W, Elo A, Nieminen K, Damen T, Joung JG, Yun JY, Lee JH, Ragni L, Reuille PB, Ahnert S, Lee JY, Mahonen AP, and Helariutta Y. Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots. Nature Plants (2019). https://doi.org/10.1038/s41477-019-0522-9
◾ Yun JY*, Kim ST, Kim SG, and Kim JS. A zero-background CRISPR binary vector system for construction of sgRNA libraries in plant functional genomics applications. Plant Biotechnology Reports (2019). https://doi.org/10.1007/s11816-019-00567-8 (*First & corresponding author)
◾ Kang BC, Yun JY*, Kim ST, Shin Y, Ryu J, Choi M, Woo JW, and Kim JS. Precision genome engineering through adenine base editing in plants. Nature Plants (2018). https://doi.org/10.1038/s41477-018-0178-x (*Co-first author)
◾ Shin SE, Lim JM, Koh HG, Kim EK, Kang NK, Jeon S, Kwon S, Shin WS, Lee B, Hwangbo K, Kim J, Ye SH, Yun JY, Seo H, Oh HM, Kim KJ, Kim JS, Jeong WJ, Chang YK and Jeong BR. CRISPR-Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii. Scientific Reports (2016). https://doi.org/10.1038/srep27810
◾ Lee J, Yun JY, Zhao W, Shen WH, and Amasino RM. A methyltransferase required for proper timing of the vernalization response in Arabidopsis. PNAS (2015). 112 (7):2269-2274
◾ Yun JY, Tamada Y, Kang YE, and Amasino RM. ARABIDOPSIS TRITHORAX-RELATED3/SET DOMAIN GROUP2 is required for repression of Arabidopsis thaliana flowering. Plant and Cell Physiology (2012). 53(5):834-846
◾ Jean Finnegan E, Bond DM, Buzas DM, Goodrich J, Helliwell CA, Tamada Y, Yun JY, Amasino RM, and Dennis ES. Polycomb proteins regulate the quantitative induction of VERNALIZATION INSENSITIVE 3 in response to low temperatures. Plant Journal (2011). 65(3):382-391
◾ Tamada Y, Yun JY, Woo SC, and Amasion RM. ARABIDOPSIS TRITHORAX-RELATED7 is required for methylation of lysine 4 of histone H3 and for transcriptional activation of FLOWERING LOCUS C. Plant Cell (2009). 21(10):3257-3269
◾ Choi J, Hyun Y, Kang MJ, In Yun H, Yun JY, Lister C, Dean C, Amasino RM, Noh B, Noh YS, and Choi Y. Resetting and regulation of FLOWERING LOCUS C expression during Arabidopsis reproductive development. Plant Journal (2009). 57(5):918-931
◾ Yun JY, Weigel D, and Lee I. Ectopic expression of SUPERMAN suppresses development of petals and stamens. Plant and Cell Physiology (2002). 43(1):52-57
Conference Papers
2021 Oral presentation at KSBS 2021
2020 Oral presentation at KMB 2020
2020 Oral presentation at KSABC 2020
2019 Oral presentation at CSH-Asia 2019 (Plant Cell and Development Biology)
2019 Oral presentation at ICAR 2019, Wuhan, China (“Omics & New Technology”)
2019 Oral presentation as an excellent lecture awardee at KSPB conference
2018 Oral presentation as a Young Investigator Research awardee at KSMCB 2018
2018 Oral presentation at IPMB 2018, Montpellier, France
2018 Oral presentation at KSABC 2018 (“Agro-Bio Genome Editing”)
2018 Oral presentation at KSPBT 2018 (“From farming to pharming”)
2016 Poster presentation at ASPB 2016, Austin, TX
2015 Oral presentation at GREEN REVOLUTION 2.0 FF21 SYMPOSIUM, UCSD
2020 Oral presentation at KMB 2020
2020 Oral presentation at KSABC 2020
2019 Oral presentation at CSH-Asia 2019 (Plant Cell and Development Biology)
2019 Oral presentation at ICAR 2019, Wuhan, China (“Omics & New Technology”)
2019 Oral presentation as an excellent lecture awardee at KSPB conference
2018 Oral presentation as a Young Investigator Research awardee at KSMCB 2018
2018 Oral presentation at IPMB 2018, Montpellier, France
2018 Oral presentation at KSABC 2018 (“Agro-Bio Genome Editing”)
2018 Oral presentation at KSPBT 2018 (“From farming to pharming”)
2016 Poster presentation at ASPB 2016, Austin, TX
2015 Oral presentation at GREEN REVOLUTION 2.0 FF21 SYMPOSIUM, UCSD
◾ 방승은, 이병하, 윤재영, X-MAS (Cross-Mediated Allele Switching), Non-Mendelian Inheritance through CRISPR-induced HDR between F1 Hybrid Alleles, 2024 KSABC International Symposium, 제주 국제 컨벤션 센터, 2024윤재영 (JAE-YOUNG YUN)
◾ Seung-eun Bang, Byeong-ha Lee, and Jae-Young Yun, X-MAS (Cross-Mediated Allele Switching), Non-Mendelian Inheritance through CRISPR-induced HDR between F1 Hybrid Alleles, 2023 KSMCB abstract book, ICC JEJU, 2023윤재영 (JAE-YOUNG YUN)
Patents
- Korea Patent pending no. 10-2021-0134852: “고올레인산 함유 종자를 생성하는 식물체 및 이의 제조 방법”
- US Patent pending no. 62/660,296/Korea Patent pending no. 10-2019-0046807: “Genome Editing through Base Editing in Plants (식물의 염기 교정용 유전자 구조체, 이를 포함하는 벡터 및 이를 이용한 염기 교정 방법)”
- US Patent pending no. 62/660,296/Korea Patent pending no. 10-2019-0046807: “Genome Editing through Base Editing in Plants (식물의 염기 교정용 유전자 구조체, 이를 포함하는 벡터 및 이를 이용한 염기 교정 방법)”
Projects
2021-2024 [NRF] CRISPR/Base Editor (BE)-dependent Library Screen for Plant Functional Genomics and Precision Breeding Technology (2021R1A2C1011674)
2020-2023 [RDA] Development of new technology for gene editing of crops (PJ016542032022)
2020-2023 [RDA] Development of new technology for gene editing of crops (PJ016542032022)
Miscellaneous
"Name one organism in the world that is not genome-editable!
"If I had to choose between DNA and RNA, I'd choose RNA because it has U in it"
"If I had to choose between DNA and RNA, I'd choose RNA because it has U in it"